In the present work, evaluation of antiulcer potential of the methanolic extract of leaves of Pongamia pinnata was done against in vivo indomethacin-induced gastric ulcer using pylorus ligation method. All the doses of the leaf extract (100, 200 and 400 mg/kg) significantly (P < 0.01) reduced the ulcer index in this study.

In Bangladesh, traditional plant-based medicines have always been used to treat hepatitis and jaundice. In the present work, we focused on medicinal plants used to treat jaundice and hepatitis. About 95 plant species belonging to about 75 family were found to be used against jaundice or hepatitis by the traditional healers. The most important plant species are Alocasia indica, Aloe barbadensis, Asparagus racemosus, Averrhoa carambola, Bixa orellana, Boerhaavia diffusa, Cucumis sativus, Cajanus Cajan, Cassia fistula, Eclipta alba, Hemidesmus indicus, Lagenaria siceraria, Mentha arvensis, Momordica charantea, Oroxylum indicum, Saccharum officinarum, Tamarindus indica etc. The ethnomedicinal inventory is presented by plant name, local name, family, chemical constituents, parts used and distribution in Bangladesh.

The purpose of this study was to characterize the putative cognitive enhancing activity of docosahexaenoic acid and gamma-linolenic acid in lead induced amnesia. PRO-PL dietary supplement containing docosahexaenoic acid and gamma-linolenic acid was administered to study cognitive enhancing activity using elevated-plus maze model and employing piracetam (200 mg/kg i.p.) as the standard drug. In the elevated–plus maze, there was significant increase in time spent and number of entries into the open arms at doses of 100 and 200 mg/kg. Dietary supplement containing docosahexaenoic acid and gamma-linolenic acid showed prominent cognitive enhancing activity in mice.

In present investigation, UV first derivative spectrophotometric methods has been developed for the determination of telmisartan in pharmaceutical formulation. The solutions of standard and sample were prepared in 0.1 M sodium hydroxide. In the UV spectrophotometric method, the quantitative determination of the drug was carried at 295 nm and the linearity range was found to be 4-20 μg/ml. For the first order derivative spectrophotometric method, the drug was determined at 311 nm with the linearity ranges 4-20 μg/ml. The calibration graphs constructed at their wavelength of determination were found to be linear for UV and derivative spectrophotometric methods. The proposed methods have been extensively validated. There was no significant difference between the performance of the proposed method regarding the mean values and standard deviations. The described method can be readily utilized for analysis of pharmaceutical formulation.

The antioxidant activity of ethanolic extract of Cassia hirsuta (Family-Caesalpiniaceae) leaves has been investigated in the present study. The antioxidant activity of the Cassia hirsuta leaves ethanolic extract was assessed by DPPH (1, 1-diphenyl-2-picrylhydrazyl) free radical scavenging method. Extract showed significant DPPH free radical scavenging effect compared with standard antioxidant ascorbic acid. IC50 value of ascorbic acid and leaves extract was found 1.25 μg/ml and 200.96 μg/ml, respectively. The value of extract indicated significant antioxidant activity of the plant.

The cytotoxic properties of ethanolic extract of Leucas aspera (Family-Lamiaceae) has been investigated in the present study. The cytotoxic potential of the L. aspera ethanolic extract was assessed by brine shrimp lethality bioassay method. Phytochemical study was done through conducting preliminary phytochemical group tests. In brine shrimp lethality bioassay, LC50 value of L. aspera ethanolic extract was found 181.68 µg/ml with 95% confidence limit where the lower and upper limits were 125.12 and 265.96 µg/ml respectively, which indicated that the extract has promising cytotoxic properties.

A rapid, sensitive and specific method for quantification of pregabalin in human plasma using imipramine as internal standard was validated. Sample preparation involved a solid phase extraction procedure. The extract was analyzed by high performance liquid chromatography coupled to electrospray tandem mass spectrometry (LC-MS-MS). Chromatography was performed isocratically on a Hypurity, 5 mu m C-18 (50 x 4.6 mm i.d.), analytical column with buffer-methanol 20:80 (v/v) as mobile phase. The response to pregabalin was a linear function of concentration over the range 250.00 to 20000.00 ng/ml. The lower limit of quantification in plasma was 250.00 ng/ml. The method was successfully applied in a bioequivalence study of a pregabalin formulation after administration single oral dose.

A 51-membered library of simple chalcones bearing a single substituent on ring-A was rationally designed. All the compounds therein were minimized using CORINA software and the target protein cyclooxygenase (COX) was normalized using CHIRON energy minimization server. Subsequently, each member of the library was docked onto an optimal, energy-minimized conformation of the human cyclooxygenase target using ArgusLab and AutoDock programs. About ten of these ligands had high docking scores, exhibited drug-likeliness and appeared to have enhanced binding to COX compared to the clinical anti-inflammatory agent - ibuprofen.

A simple, specific, accurate and precise reverse phase high performance liquid chromatographic method is developed and validated for the estimation of telmisartan in tablet dosage form. The expected separation and peak shapes were obtained on chromosil C18 (250 mm x 4.6 mm, 5 μm) column. To have an ideal separation of the drug under isocratic conditions, mixtures of solvents like methanol, water and acetonitrile with or without different buffers in different combinations were tested as mobile phases on a chromosil C18 column. A mixture of methanol : 0.1% orthophosphoric acid : acetonitrile in the ratio of 80:05:15 v/v/v was proved to be the most suitable of all the combinations since the chromatographic peak obtained was better defined and resolved and was almost free from tailing. The flow rate was 1.5 ml/min and effluents were monitored at 256 nm. The retention time for telmisartan was 2.7 min. The method was validated and found to be accurate, and precise. Recovery of telmisartan from tablet formulation was found to be 99.41%. The proposed method was successfully applied for the quantitative determination of telmisartan in tablet formulation.

In present study, a novel series of trisubstituted phenoxyacetyl amino acids and peptide derivatives was synthesized by coupling 2-(2,6-dibromo-4-formylphenoxy)acetic acid with amino acid methyl ester hydrochlorides/peptide methyl esters using DIPC as coupling agent and TEA as base. The structures were elucidated by IR, 1H NMR, 13C NMR and MS spectral data as well as elemental analysis. The newly synthesized peptide derivatives were evaluated for antibacterial and antifungal potential against pathogenic microorganisms. Compounds Ig, Ik and In displayed potent antibacterial activity against gram-negative bacteria Pseudomonas aeruginosa, Escherichia coli and compounds Ic, Ih, Il and Ij, Im were found to exhibit potent antifungal activity against pathogenic Candida albicans and dermatophytes, as compared to standard drugs ciprofloxacin and griseofulvin.